in vitro Fertilization
The 5th International Symposium on Pacific squid (9-15 October 2010) was held in La Paz, Mexico and was hosted by the Centro de Investigaciones Biológicas del Noroeste (CIBNOR). It included a workshop entitled "Considerations for in vitro embryonic development in Dosidicus gigas: theory and practice", kindly organized by Susana Camarillo-Coop and César A. Salinas-Zavala. See pictures from the workshop here.
The following scientists participated in the workshop: Juan Argüelles, Susana Camarillo-Coop, Chingis M. Nigmatullin, Mitsuo Sakai, Yasunori Sakurai, Danna J. Staaf, César A. Salinas-Zavala, Roxana De Silva-Dávila, Ricardo Tafur, Érica A.G. Vidal, Roger Villanueva, Carmen Yamashiro.
As a conclusion of the workshop, participants compiled their published and unpublished methodologies into a user-friendly “cooking recipe” to be used in future research. View here the resulting publication of "A laboratory guide to in vitro fertilization of oceanic squids". The following figures describe these methods and protocols.
Figure 1. Schematic sequence of laboratory steps for the gamete collection, in vitro fertilization and egg incubation of oceanic squid. FSW, filtered seawater; FSW+A, filtered seawater with antibiotics.
Figure 2. Reproductive anatomy of Ommastrephid squids. A, mature female Dosidicus gigas; B, mature female Illex coindetii (note that nidamental glands have been displaced laterally to shown the spermatangia); C, mature male Dosidicus gigas; D, mature male Illex argentinus. Abbreviations: ng, nidamental gland; od, oviduct; og, oviducal gland; ov, ovary; sg, spermatophoric gland; sp, spermatophores (released); spg, spermatangia; ss, spermatophoric sac; to, terminal organ (passes under the gill); ts, testis; vd, vas deferens.
Both figures from: Villanueva, R., Staaf, D.J., Argüelles, J., Bozzano, A., Camarillo-Coop, S., Nigmatullin, C.M., Petroni, G., Quintana, D., Sakai, M., Sakurai, Y., Salinas-Zavala, C.A. De Silva-Dávila, R.,Tafur, R., Yamashiro, C., Vidal, E.A.G. 2012. A laboratory guide to in vitro fertilization of oceanic squids. Aquaculture, 342-343: 125-133. View PDF.